解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Protoporphyrin IX (PpIX)-encapsulated mesoporous silica nanoparticles were synthesized, characterized, and utilized for photodynamic therapy (PDT) of cancer. Silica encapsulation is relatively transparent for activated light and can protect the PpIX against denaturation induced by the extreme bioenvironment. The mesop...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3083427
更新日期:2009-01-01 00:00:00
abstract::The essential feature of the confocal laser scanning microscope (cLSM) is the generation of optical sections by the removal of out-of-focus light. About ten years ago, structured illumination microscopy (SIM) was introduced as an alternative method for obtaining optical sections from biological specimens. Here we comp...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3083439
更新日期:2009-01-01 00:00:00
abstract::We present in vivo human total retinal blood flow measurements using Doppler Fourier domain optical coherence tomography (OCT). The scan pattern consisted of two concentric circles around the optic nerve head, transecting all retinal branch arteries and veins. The relative positions of each blood vessel in the two OCT...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2998480
更新日期:2008-11-01 00:00:00
abstract::Spatially resolved spectroscopy (SRS) allows the estimation of absolute tissue oxygen saturation, the ratio of oxygenated to total hemoglobin concentration, which may facilitate the comparison of results among patients. Eighty-two premature infants were included over two years. The cerebral tissue oxygenation index (c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3013454
更新日期:2008-11-01 00:00:00
abstract::We report the design of a novel laser line-triangulation laryngoscope for the quantitative visualization of the three-dimensional movements of human vocal folds during phonation. This is the first successful in vivo recording of the three-dimensional movements of human vocal folds in absolute values. Triangulation ima...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3041164
更新日期:2008-11-01 00:00:00
abstract::In order to evaluate the impact of anatomy on the spectral properties of oral tissue, we used reflectance and fluorescence spectroscopy to characterize nine different anatomic sites. All spectra were collected in vivo from healthy oral mucosa. We analyzed 710 spectra collected from the oral cavity of 79 healthy volunt...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2992139
更新日期:2008-11-01 00:00:00
abstract::An integrated miniature multi-modal microscope (4M device) for microendoscopy was built and tested. Imaging performance is evaluated and imaging results are presented for both fluorescence and reflectance samples. Images of biological samples show successful imaging of both thin layers of fixed cells prepared on a sli...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2978060
更新日期:2008-09-01 00:00:00
abstract::We present a study using a method able to assess tissue oxygenation, taking into account the absorption and the level of scattering in myocardial tissue using a calibrated fiber optic probe. With this method, interindividual comparisons of oxygenation can be made despite varying tissue optical properties during corona...
journal_title:Journal of biomedical optics
pub_type: 临床试验,杂志文章
doi:10.1117/1.2976433
更新日期:2008-09-01 00:00:00
abstract::Diffusion of therapeutic macromolecules through the extracellular matrix of tumor tissue is a crucial step in drug delivery. We use fluorescence correlation spectroscopy (FCS) to measure diffusion of IgG (150 kDa) and dextrans (155 kDa and 2 MDa) in solution, 5% gelatin hydrogel, and multicellular spheroids. Gel and s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2982530
更新日期:2008-09-01 00:00:00
abstract::The fibrillar collagen network in tumor and normal tissues is different due to remodeling of the extracellular matrix during the malignant process. Collagen type I fibers have the crystalline and noncentrosymmetric properties required for generating the second-harmonic signal. The content and structure of collagen wer...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2983664
更新日期:2008-09-01 00:00:00
abstract::Treatment of deep venous thrombosis (DVT)--a primary cause of potentially fatal pulmonary embolism (PE)--depends on the age of the thrombus. The existing clinical imaging methods are capable of visualizing a thrombus but cannot determine the age of the blood clot. Therefore, there is a need for an imaging technique to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2992175
更新日期:2008-09-01 00:00:00
abstract::A real-time photoacoustic imaging system is designed and built. This system is based on a commercially available ultrasound imaging system. It can achieve a frame rate of 8 frames/sec. Vasculature in the hand of a human volunteer is imaged, and the resulting photoacoustic image is combined with the ultrasound image. T...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3005421
更新日期:2008-09-01 00:00:00
abstract::We instrumented a combined fluorescence spectroscopy and imaging system to characterize the single- and two-photon excited autofluorescence in epithelial tissue. Single-photon fluorescence (SPF) are compared with two-photon fluorescence (TPF) measured at the same location in epithelial tissue. It was found that the SP...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2975866
更新日期:2008-09-01 00:00:00
abstract::A tutorial introduction to diffuse light transport is presented. The basic analytic equations of time-resolved, steady-state and modulated light transport are introduced. The perturbation method for handling slight heterogeneities in optical properties is outlined. The treatment of boundary conditions such as an air/t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2967535
更新日期:2008-07-01 00:00:00
abstract::Current techniques to assess lymph node metastases in cancer patients include lymphoscintigraphy after administration of a nonspecific radiocolloid in order to locate and resect lymph nodes for pathological examination of harbored cancer cells. Clinical trials involving intradermal or subcutaneous injection of antibod...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.2953498
更新日期:2008-07-01 00:00:00
abstract::Shallow lesions less than 1.5-cm deep are frequently seen in breast patients when they are scanned in reflection geometry. Two boundary conditions are compared for imaging shallow lesions, and a new probe design is introduced. A partial reflection boundary condition is suitable for imaging shallow lesions less than 1....
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2966703
更新日期:2008-07-01 00:00:00
abstract::We demonstrate the ex vivo imaging of an oral cancerous sample with a swept-source optical coherence tomography (SS-OCT) system. With the axial resolution of 8 microm in free space and system sensitivity of 108 dB, we can well distinguish the normal and abnormal tissue portions in a sample. In particular, we analyze t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2960632
更新日期:2008-07-01 00:00:00
abstract::We used two-photon laser scanning microscopy (TPLSM) to demonstrate for the first time its potential in studying relational details at the cellular level of atherogenesis in intact, viable mouse carotid arteries. Isolated and mounted arteries of ApoE-/-mice, aged 15 or 21 weeks (7 and 13 weeks on western diet), were i...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2965542
更新日期:2008-07-01 00:00:00
abstract::Temperature distribution is a crucial factor in determining the outcome of laser phototherapy in cancer treatment. Magnetic resonance imaging (MRI) is an ideal method for 3-D noninvasive temperature measurement. A 7.1-T MRI was used to determine laser-induced high thermal gradient temperature distribution of target ti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2960020
更新日期:2008-07-01 00:00:00
abstract::We report that the pathologic components present within the atheromatous plaques of ApoE knock-out mice can reflect significant amounts of mid-infrared (mid-IR) light. Furthermore, the reflected light spectra contained the unique signatures of a variety of biologic features including those found in unstable or "vulner...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2937469
更新日期:2008-05-01 00:00:00
abstract::The recycling of G-protein-coupled receptors (GPCR) to the cell surface after internalization plays an important role in the regulation of overall GPCR activity. The angiotensin II type I receptor (AT1R) belongs to class B GPCRs that recycle slowly back to the cell surface. Previous studies have proposed that Rab11 co...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2943286
更新日期:2008-05-01 00:00:00
abstract::Using single-molecule microscopy, we present a method to quantify the number of single autofluorescent proteins when they cannot be optically resolved. This method relies on the measurement of the total intensity emitted by each aggregate until it photobleaches. This strategy overcomes the inherent problem of blinking...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2940600
更新日期:2008-05-01 00:00:00
abstract::Since 1978 in vitro fertilization (IVF) procedures have resulted in the birth of over 3 million babies. Yet in 2005, IVF procedures had a live birth rate of only 34%, with 32% of these births resulting in multiple pregnancies. These multiple pregnancies were directly attributed to the transfer of multiple embryos to i...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2937468
更新日期:2008-05-01 00:00:00
abstract::We explore the use of Monte-Carlo-model-based approaches for the analysis of fluorescence and diffuse reflectance spectra measured ex vivo from breast tissues. These models are used to extract the absorption, scattering, and fluorescence properties of malignant and nonmalignant tissues and to diagnose breast cancer ba...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2931078
更新日期:2008-05-01 00:00:00
abstract::Artificially created demineralized and remineralized carious lesions on the root and enamel of human teeth were examined by photothermal radiometry (PTR) and modulated luminescence (LUM). Fourteen extracted human teeth were used and a lesion was created on a 1 mmx4 mm rectangular window, spanning root to enamel, using...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2942374
更新日期:2008-05-01 00:00:00
abstract::Optical clearing is a well-known phenomenon. It is based on the matching of refractive indices of a bulk material and scattering particles. The same principle is also used in scattering-based optical measurements of different constituents, such as glucose. By registering changes in scattering, it is possible to evalua...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2904957
更新日期:2008-03-01 00:00:00
abstract::We present systematic characterization of a photoacoustic imaging system optimized for rapid, high-resolution tomographic imaging of small animals. The system is based on a 128-element ultrasonic transducer array with a 5-MHz center frequency and 80% bandwidth shaped to a quarter circle of 25 mm radius. A 16-channel d...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2907157
更新日期:2008-03-01 00:00:00
abstract::A Monte-Carlo-based model of fluorescence is developed that is capable of extracting the intrinsic fluorescence properties of tissue, which are independent of the absorption and scattering properties of tissue. This model is flexible in its applicability to different illumination-collection geometries and is also vali...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2907161
更新日期:2008-03-01 00:00:00
abstract::Noninvasive cardiovascular imaging could lead to the early detection and timely treatment of complex atherosclerotic lesions responsible for major cardiovascular events. Recent investigations have suggested that optical coherence tomography (OCT) is an ideal diagnostic tool due to the high resolution this technology a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2870153
更新日期:2008-01-01 00:00:00
abstract::We combine laser tweezers with custom computer tracking software and robotics to analyze the motility [swimming speed, VCL (curvilinear velocity), and swimming force in terms of escape laser power (Pesc)] and energetics [mitochondrial membrane potential (MP)] of individual sperm. Domestic dog sperm are labeled with a ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2839051
更新日期:2008-01-01 00:00:00
abstract::We have been developing a Mach-Zehnder type of optical frequency domain reflectometry optical coherence tomography (OFDR-OCT) that uses discretely swept superstructure-grating distributed Bragg- reflector (SSG-DBR) lasers developed for telecommunication fields and has a 12-mm-depth range. We report images obtained wit...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2839042
更新日期:2008-01-01 00:00:00
abstract::We present a study using plasmonic nanoparticles (NPs) to image epidermal growth factor receptor (EGFR) in live cells. Through detailed analysis of the NP scattering spectra, we determine the intracellular refractive index (RI) within attoliter volumes inside of the living cells. Molecular imaging is demonstrated usin...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2837450
更新日期:2008-01-01 00:00:00
abstract::By using a novel temporal characterization technique, we determined that a threshold average laser power of 160 mW is required to drill through a 0.75-mm-thick cortical bone for a Nd:YVO(4) mode-locked laser oscillator with a peak intensity of 1.3 GWcm(2). The ablation mechanism is identified as average-power induced ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2821149
更新日期:2007-11-01 00:00:00
abstract::This work reviews our research during the past several years on time-resolved (TR) near-infrared diffuse optical tomography (DOT). Following an introduction of the measuring modes, two proposed schemes of image reconstruction in TR-DOT are described: one utilizes the full TR data, and the other, referred to as the mod...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.2815724
更新日期:2007-11-01 00:00:00
abstract::A Monte Carlo algorithm is applied to simulate the measurements of a sample with glass slides sandwiched between the double integrating sphere (DIS) setup. The effects caused by various parameters, such as the sample port of integrating sphere, thicknesses, and optical properties of the sample, on light losses and opt...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2815691
更新日期:2007-11-01 00:00:00
abstract::Our purpose is to evaluate intramucosal gastric pH video imaging by 2('),7(')-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorescence ratio techniques. We use a video endoscopic imaging system and BCECF as the pH fluorescent probe. Systemic in vivo pH variations are studied in 10 pigs: five in the control group ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2821698
更新日期:2007-11-01 00:00:00
abstract::Full-field optical coherence tomography (FF-OCT) capable of in vivo cellular-level imaging is demonstrated for nonscanning horizontal cross-sectional imaging. The system is based on a white light interference microscope illuminated by a thermal light source. A dual-channel two-dimensional (2-D) detection technique inc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2822159
更新日期:2007-11-01 00:00:00
abstract::Selective laser targeting of the retinal pigment epithelium (RPE) is an attractive method for treating RPE-associated disorders. We are developing a method for optically detecting intracellular microcavitation that can potentially serve as an immediate feedback of the treatment outcome. Thermal denaturation or intrace...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2804078
更新日期:2007-11-01 00:00:00
abstract::We present the first experimental result of direct delineation of the nuclei of living rat bladder epithelium with ultrahigh-resolution optical coherence tomography (uOCT). We demonstrate that the cellular details embedded in the speckle noise in a uOCT image can be uncovered by time-lapse frame averaging that takes a...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.2800007
更新日期:2007-09-01 00:00:00
abstract::In a previous study, we investigated physical methods to reduce whole-body, diet-related autofluorescence interference in several mouse strains through changes in animal diet. Measurements of mice with an in vivo multispectral imaging system over a 21-day period allowed for the quantification of concentration changes ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2799188
更新日期:2007-09-01 00:00:00